[The generation and characteristics of reassortant influenza A virus with H5 hemagglutinin and other genes from the apathogenic virus H6N2].
Identifieur interne : 002D80 ( Main/Exploration ); précédent : 002D79; suivant : 002D81[The generation and characteristics of reassortant influenza A virus with H5 hemagglutinin and other genes from the apathogenic virus H6N2].
Auteurs : E Iu Boravleva ; N F Lomakina ; E A Kropotkina ; I A Rudneva ; S S Iamnikova ; L G Rudenko ; V V Drygin ; A S GambarianSource :
- Voprosy virusologii [ 0507-4088 ]
Descripteurs français
- KwdFr :
- Animaux, Anticorps antiviraux (biosynthèse), Anticorps antiviraux (immunologie), Charadriiformes (immunologie), Embryon de poulet, Glycoprotéine hémagglutinine du virus influenza (), Grippe chez les oiseaux (), Grippe chez les oiseaux (immunologie), Grippe humaine (), Grippe humaine (immunologie), Humains, Modèles animaux, Poulets (immunologie), Réplication virale, Souris, Sous-type H5N2 du virus de la grippe A (génétique), Sous-type H5N2 du virus de la grippe A (immunologie), Température, Vaccination, Vaccins antigrippaux (génétique), Vaccins antigrippaux (immunologie), Vaccins atténués (génétique), Vaccins atténués (immunologie), Virus recombinants (génétique), Virus recombinants (immunologie).
- MESH :
- biosynthèse : Anticorps antiviraux.
- génétique : Sous-type H5N2 du virus de la grippe A, Vaccins antigrippaux, Vaccins atténués, Virus recombinants.
- immunologie : Anticorps antiviraux, Charadriiformes, Grippe chez les oiseaux, Grippe humaine, Poulets, Sous-type H5N2 du virus de la grippe A, Vaccins antigrippaux, Vaccins atténués, Virus recombinants.
- Animaux, Embryon de poulet, Glycoprotéine hémagglutinine du virus influenza, Grippe chez les oiseaux, Grippe humaine, Humains, Modèles animaux, Réplication virale, Souris, Température, Vaccination.
English descriptors
- KwdEn :
- Animals, Antibodies, Viral (biosynthesis), Antibodies, Viral (immunology), Charadriiformes (immunology), Chick Embryo, Chickens (immunology), Hemagglutinin Glycoproteins, Influenza Virus (chemistry), Humans, Influenza A Virus, H5N2 Subtype (genetics), Influenza A Virus, H5N2 Subtype (immunology), Influenza Vaccines (genetics), Influenza Vaccines (immunology), Influenza in Birds (immunology), Influenza in Birds (prevention & control), Influenza, Human (immunology), Influenza, Human (prevention & control), Mice, Models, Animal, Reassortant Viruses (genetics), Reassortant Viruses (immunology), Temperature, Vaccination, Vaccines, Attenuated (genetics), Vaccines, Attenuated (immunology), Virus Replication.
- MESH :
- chemical , biosynthesis : Antibodies, Viral.
- chemical , chemistry : Hemagglutinin Glycoproteins, Influenza Virus.
- chemical , genetics : Influenza Vaccines, Vaccines, Attenuated.
- chemical , immunology : Antibodies, Viral, Influenza Vaccines, Vaccines, Attenuated.
- genetics : Influenza A Virus, H5N2 Subtype, Reassortant Viruses.
- immunology : Charadriiformes, Chickens, Influenza A Virus, H5N2 Subtype, Influenza in Birds, Influenza, Human, Reassortant Viruses.
- prevention & control : Influenza in Birds, Influenza, Human.
- Animals, Chick Embryo, Humans, Mice, Models, Animal, Temperature, Vaccination, Virus Replication.
Abstract
The experimental reassortant vaccine strain VN-gull (H5N2) containing H5 hemagglutinin (HA) with a removed polybasic site in the connecting peptide and other genes from the apathogenic H6N2 virus A/gull/Moscow/3100/2006 (gull/M) was obtained using a two-step protocol. At Step 1, the reassortant with HA of A/Vietnam/1203/04-PR8/ CDC-RG and other genes from cold-adapted A/Leningrad/17/47 (VN-Len) viruses was generated due to selection with antibody to H2N2 at 26 degrees C. At Step 2, the reassortant VN-gull was obtained by replacing all genes from Len with those from gull/M due to selection with antibody to H6N2 at 39 degrees C. The reassortant VN-Len was apathogenic and the reassortant VN-gull was weakly virulent in mice. Both gave rise to specific antibodies and 4 weeks after single inoculation they provided complete protection against further challenge with highly pathogenic HSN1 virus A/chicken/Kurgan/3/05 (H5N1) (Ku-Len). The chickens infected with live VN-gull virus showed neither clinical symptoms, nor fecal virus excretion; nevertheless, they gave rise to antibodies and were protected from the further challenge with A/chicken/Kurgan/3/2005. The high yield, safety, and protectivity of VN-Len and Ku-Len made them promising strains for the production of inactivated and live vaccines against H5N1 viruses.
PubMed: 22359942
Affiliations:
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Le document en format XML
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(biosynthèse)</term><term>Anticorps antiviraux (immunologie)</term><term>Charadriiformes (immunologie)</term><term>Embryon de poulet</term><term>Glycoprotéine hémagglutinine du virus influenza ()</term><term>Grippe chez les oiseaux ()</term><term>Grippe chez les oiseaux (immunologie)</term><term>Grippe humaine ()</term><term>Grippe humaine (immunologie)</term><term>Humains</term><term>Modèles animaux</term><term>Poulets (immunologie)</term><term>Réplication virale</term><term>Souris</term><term>Sous-type H5N2 du virus de la grippe A (génétique)</term><term>Sous-type H5N2 du virus de la grippe A (immunologie)</term><term>Température</term><term>Vaccination</term><term>Vaccins antigrippaux (génétique)</term><term>Vaccins antigrippaux (immunologie)</term><term>Vaccins atténués (génétique)</term><term>Vaccins atténués (immunologie)</term><term>Virus recombinants (génétique)</term><term>Virus recombinants (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" 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Replication</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Embryon de poulet</term><term>Glycoprotéine hémagglutinine du virus influenza</term><term>Grippe chez les oiseaux</term><term>Grippe humaine</term><term>Humains</term><term>Modèles animaux</term><term>Réplication virale</term><term>Souris</term><term>Température</term><term>Vaccination</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The experimental reassortant vaccine strain VN-gull (H5N2) containing H5 hemagglutinin (HA) with a removed polybasic site in the connecting peptide and other genes from the apathogenic H6N2 virus A/gull/Moscow/3100/2006 (gull/M) was obtained using a two-step protocol. At Step 1, the reassortant with HA of A/Vietnam/1203/04-PR8/ CDC-RG and other genes from cold-adapted A/Leningrad/17/47 (VN-Len) viruses was generated due to selection with antibody to H2N2 at 26 degrees C. At Step 2, the reassortant VN-gull was obtained by replacing all genes from Len with those from gull/M due to selection with antibody to H6N2 at 39 degrees C. The reassortant VN-Len was apathogenic and the reassortant VN-gull was weakly virulent in mice. Both gave rise to specific antibodies and 4 weeks after single inoculation they provided complete protection against further challenge with highly pathogenic HSN1 virus A/chicken/Kurgan/3/05 (H5N1) (Ku-Len). The chickens infected with live VN-gull virus showed neither clinical symptoms, nor fecal virus excretion; nevertheless, they gave rise to antibodies and were protected from the further challenge with A/chicken/Kurgan/3/2005. The high yield, safety, and protectivity of VN-Len and Ku-Len made them promising strains for the production of inactivated and live vaccines against H5N1 viruses.</div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Boravleva, E Iu" sort="Boravleva, E Iu" uniqKey="Boravleva E" first="E Iu" last="Boravleva">E Iu Boravleva</name><name sortKey="Drygin, V V" sort="Drygin, V V" uniqKey="Drygin V" first="V V" last="Drygin">V V Drygin</name><name sortKey="Gambarian, A S" sort="Gambarian, A S" uniqKey="Gambarian A" first="A S" last="Gambarian">A S Gambarian</name><name sortKey="Iamnikova, S S" sort="Iamnikova, S S" uniqKey="Iamnikova S" first="S S" last="Iamnikova">S S Iamnikova</name><name sortKey="Kropotkina, E A" sort="Kropotkina, E A" uniqKey="Kropotkina E" first="E A" last="Kropotkina">E A Kropotkina</name><name sortKey="Lomakina, N F" sort="Lomakina, N F" uniqKey="Lomakina N" first="N F" last="Lomakina">N F Lomakina</name><name sortKey="Rudenko, L G" sort="Rudenko, L G" uniqKey="Rudenko L" first="L G" last="Rudenko">L G Rudenko</name><name sortKey="Rudneva, I A" sort="Rudneva, I A" uniqKey="Rudneva I" first="I A" last="Rudneva">I A Rudneva</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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